Adam,
I loved your explanation of the flat dark. I am thinking of ways to check dark current accumulation and the need for a flat-dark versus a bias.
Assumptions:
1. If you subtract a bias from a bias I would expect a clean frame with ADU counts at or near 0. Correct?
2. so if you are worried about whether you can get away with only calibrating your flats with a master bias, could you not subtract your 10 second or 30 second flat-dark from a bias and judge the level of the dark current by checking the average ADU could of the resulting frame?
If so, then what you would need is an algorithm to test whether the residual ADU count was significant relative to the quality of your flat.
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